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rabbit polyclonal anti p2x4r antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal anti p2x4r antibody
    Rabbit Polyclonal Anti P2x4r Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p2x4/Anti-P2X4+Receptor+Antibody/pm41034045-33-0-24
    Average 95 stars, based on 100 article reviews
    rabbit polyclonal anti p2x4r antibody - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: EXPRESS: Humanized anti-P2X4 scFv Reduces ATP-Induced P2X4 Currents and Modulates Excitability in Human DRG Neurons
    Article Snippet: .. The sections were incubated at room temperature in a humidified chamber on a rocking table with the following primary antibodies and dilutions in blocking solution: chicken anti-peripherin (PA1-10012, Invitrogen, 1:2000) and rabbit anti-P2X4 (APR-002, Alomone Labs, 1:500). .. Primary anti-body solution was gently pipetted out of wells before being rinsed 5x in 1X TBS, followed by a 2-hour incubation at room temperature in secondary antibodies in 1X TBS: goat anti-chicken AF555 (AB150170, Abcam, 1:2000) and donkey anti-rabbit AF488 (A21206, Invitrogen, 1:2000).

    Article Title: Humanized anti-P2X4 scFv reduces ATP-induced P2X4 currents and modulates excitability in human DRG neurons
    Article Snippet: .. The sections were incubated at room temperature in a humidified chamber on a rocking table with the following primary antibodies and dilutions in blocking solution: chicken anti-peripherin (PA1-10012, Invitrogen, 1:2000) and rabbit anti-P2X4 (APR-002, Alomone Labs, 1:500). .. The sections were incubated at room temperature in a humidified chamber on a rocking table with the following primary antibodies and dilutions in blocking solution: chicken anti-peripherin (PA1-10012, Invitrogen, 1:2000) and rabbit anti-P2X4 (APR-002, Alomone Labs, 1:500).

    Article Title: Dorsal root ganglia P2X4 and P2X7 receptors contribute to diabetes-induced hyperalgesia and the downregulation of electroacupuncture on P2X4 and P2X7
    Article Snippet: .. They were then incubated overnight with rabbit anti-P2X4 (1:200, APR-002, Alomone, Israel) and rabbit anti-P2X7 (1:500, GTX104288, Gene Tex, USA) at 4 °C. ..

    Article Title: The P2X7 Hypothesis of Central Post-Stroke Pain
    Article Snippet: Proteins were separated via 6–8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; 6% SDS-PAGE for NKCC1 separation) and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). .. After blocking with 5% skim milk/TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies diluted in 0.5% nonfat skim milk/TBST: rabbit anti-KCC2 (1:1000, Millipore), rabbit anti-NKCC1 (1:400, Abcam, Hong Kong, China), rabbit anti-TrkB (1:1000, Abcam), rabbit anti-GABAa a2 subunit (1:1000, Abcam), rabbit anti-P2X4 (1:400, APR-002, Alomone, Jerusalem, Israel), and mouse anti-tubulin (1:2500, Sigma). ..

    Article Title: Neuronal P2X4 receptor may contribute to peripheral inflammatory pain in rat spinal dorsal horn
    Article Snippet: The membranes were blocked with 10% normal bovine serum albumin (BSA, Sigma-Aldrich) in Tween-Tris-buffered saline (TTBS solution, 20 mM TRIS, 500 mM NaCl, pH 7.5, 0.05% Tween-20). .. Membranes were incubated with rabbit anti-P2X4 (1:1,000, Alomone Labs) and internal control antibody (mouse anti-β-tubulin, 1:2,000, Sigma-Aldrich) for 2 hours room temperature. .. Upon washing with TTBS, membranes were treated with goat anti-rabbit secondary antibody conjugated with horse-radish-peroxidase, (HRP, 1:200, DakoCytomation, Glostrup, Denmark) and goat anti-mouse secondary antibody conjugated with HRP (1:200, DakoCytomation).

    Blocking Assay:

    Article Title: EXPRESS: Humanized anti-P2X4 scFv Reduces ATP-Induced P2X4 Currents and Modulates Excitability in Human DRG Neurons
    Article Snippet: .. The sections were incubated at room temperature in a humidified chamber on a rocking table with the following primary antibodies and dilutions in blocking solution: chicken anti-peripherin (PA1-10012, Invitrogen, 1:2000) and rabbit anti-P2X4 (APR-002, Alomone Labs, 1:500). .. Primary anti-body solution was gently pipetted out of wells before being rinsed 5x in 1X TBS, followed by a 2-hour incubation at room temperature in secondary antibodies in 1X TBS: goat anti-chicken AF555 (AB150170, Abcam, 1:2000) and donkey anti-rabbit AF488 (A21206, Invitrogen, 1:2000).

    Article Title: Humanized anti-P2X4 scFv reduces ATP-induced P2X4 currents and modulates excitability in human DRG neurons
    Article Snippet: .. The sections were incubated at room temperature in a humidified chamber on a rocking table with the following primary antibodies and dilutions in blocking solution: chicken anti-peripherin (PA1-10012, Invitrogen, 1:2000) and rabbit anti-P2X4 (APR-002, Alomone Labs, 1:500). .. The sections were incubated at room temperature in a humidified chamber on a rocking table with the following primary antibodies and dilutions in blocking solution: chicken anti-peripherin (PA1-10012, Invitrogen, 1:2000) and rabbit anti-P2X4 (APR-002, Alomone Labs, 1:500).

    Article Title: The P2X7 Hypothesis of Central Post-Stroke Pain
    Article Snippet: Proteins were separated via 6–8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; 6% SDS-PAGE for NKCC1 separation) and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). .. After blocking with 5% skim milk/TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies diluted in 0.5% nonfat skim milk/TBST: rabbit anti-KCC2 (1:1000, Millipore), rabbit anti-NKCC1 (1:400, Abcam, Hong Kong, China), rabbit anti-TrkB (1:1000, Abcam), rabbit anti-GABAa a2 subunit (1:1000, Abcam), rabbit anti-P2X4 (1:400, APR-002, Alomone, Jerusalem, Israel), and mouse anti-tubulin (1:2500, Sigma). ..

    Western Blot:

    Article Title: Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer’s disease
    Article Snippet: Immunocytochemistry goat anti-ApoE (1:1000, Millipore, AB947), goat anti-Cathepsin B (1:1000, R&D systems, AF965), rabbit anti-P2X4 (1:200, Sigma, HPA039494), donkey anti-CD68 (1:300, Biorad, MCA1957A488T), mouse anti-6E10 (1:500, Biolegend, SIG-39320–0200), rabbit anti-Iba1 (Ionized calcium-binding adapter molecule 1, 1:2000, Wako, MNK4428), rat anti-P2X4 (1:200, kindly provided by Dr. Nolte [ ]), donkey anti-rat-A594 (1:500, Jackson Immunoresearch, 712-586-150), donkey anti-goat-A488 (1:2000, Molecular probes, A11055), donkey anti-rabbit-A557 (1:2000, R&D systems, NL004), goat anti-rabbit-A488 (1:2000, Molecular probes, A11034). .. Western blot mouse anti-tubulin (1:5000, Sigma, T9026), rabbit anti-HA (1:500, Invitrogen, 715,500), rabbit anti-P2X4 (1:500, Alomone Labs, APR-002), anti-HA beads (1:500 Santa-Cruz Biotechnology), horse anti-mouse-HRP (1:2000, Cell signaling, 7076S), goat anti-rabbit-HRP (1:2000, Jackson Immunoresearch, 111-035-144), donkey anti-goat-HRP (1:2000, Jackson Immunoresearch, 705–035-003). .. For BMDM culture cells, supernatants were collected in Amicon column (Millipore, UFC5010BK) and centrifuged at 14,000g for 30 min at 4 °C.

    Control:

    Article Title: Neuronal P2X4 receptor may contribute to peripheral inflammatory pain in rat spinal dorsal horn
    Article Snippet: The membranes were blocked with 10% normal bovine serum albumin (BSA, Sigma-Aldrich) in Tween-Tris-buffered saline (TTBS solution, 20 mM TRIS, 500 mM NaCl, pH 7.5, 0.05% Tween-20). .. Membranes were incubated with rabbit anti-P2X4 (1:1,000, Alomone Labs) and internal control antibody (mouse anti-β-tubulin, 1:2,000, Sigma-Aldrich) for 2 hours room temperature. .. Upon washing with TTBS, membranes were treated with goat anti-rabbit secondary antibody conjugated with horse-radish-peroxidase, (HRP, 1:200, DakoCytomation, Glostrup, Denmark) and goat anti-mouse secondary antibody conjugated with HRP (1:200, DakoCytomation).



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